About the Project

In the lower Chesapeake Bay, Virginia, warmer water temperatures in recent years have resulted in large scale diebacks of eelgrass meadows (Zostera marina). In contrast, many eelgrass populations in Back Sound, North Carolina appear to be more resilient to warming water temperatures. Understanding the drivers of these regional differences in eelgrass resilience could help more effectively restore eelgrass meadows in a changing climate. With a network of the intended users from reserves, state agencies, and Chesapeake Bay nonprofits, this project compared resiliency traits of eelgrass populations in Virginia and North Carolina by conducting reciprocal restoration trials and genomic sequencing. The project results indicate the importance of seed sources in potential future eelgrass restoration, in addition to site selection.

About this Resource

This resource is a detailed laboratory procedure for isolating high-quality DNA from Zostera marina (eelgrass) tissue using a modified CTAB (cetyltrimethylammonium bromide) method. The protocol outlines reagent preparation, tissue processing, incubation steps, organic extractions, and DNA precipitation using isopropanol. It includes guidance on troubleshooting issues related to polysaccharides, optimizing spin conditions, and maximizing DNA yield and purity. The document also offers practical advice on resuspension, storage, and safe disposal of hazardous waste.

This protocol is intended for researchers conducting genetic or genomic analyses on seagrasses or other marine plants. It is designed for users with basic to intermediate laboratory experience in molecular biology, particularly those conducting genetic or genomic studies requiring high-quality DNA. The protocol is also useful for restoration scientists and graduate students involved in population genetics, conservation genomics, or ecological monitoring programs where DNA extraction from plant tissues is a foundational step.